Transient Protein Expression by Agroinfiltration in Lettuce.
Identifieur interne : 000261 ( Main/Exploration ); précédent : 000260; suivant : 000262Transient Protein Expression by Agroinfiltration in Lettuce.
Auteurs : Qiang Chen [États-Unis] ; Matthew Dent [États-Unis] ; Jonathan Hurtado [États-Unis] ; Jake Stahnke [États-Unis] ; Alyssa Mcnulty [États-Unis] ; Kahlin Leuzinger [États-Unis] ; Huafang Lai [États-Unis]Source :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2016.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Agrobacterium tumefaciens, Laitue.
- métabolisme : Laitue.
- méthodes : Génie génétique.
- Expression des gènes, Protéines recombinantes, Transformation génétique, Vecteurs génétiques, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- MESH :
- chemical : Recombinant Proteins.
- genetics : Agrobacterium tumefaciens, Lettuce.
- metabolism : Lettuce.
- methods : Genetic Engineering.
- Gene Expression, Genetic Vectors, Plants, Genetically Modified, Transformation, Genetic.
Abstract
Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.
DOI: 10.1007/978-1-4939-3289-4_4
PubMed: 26614281
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Transient Protein Expression by Agroinfiltration in Lettuce.</title><author><name sortKey="Chen, Qiang" sort="Chen, Qiang" uniqKey="Chen Q" first="Qiang" last="Chen">Qiang Chen</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Dent, Matthew" sort="Dent, Matthew" uniqKey="Dent M" first="Matthew" last="Dent">Matthew Dent</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Hurtado, Jonathan" sort="Hurtado, Jonathan" uniqKey="Hurtado J" first="Jonathan" last="Hurtado">Jonathan Hurtado</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Stahnke, Jake" sort="Stahnke, Jake" uniqKey="Stahnke J" first="Jake" last="Stahnke">Jake Stahnke</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Mcnulty, Alyssa" sort="Mcnulty, Alyssa" uniqKey="Mcnulty A" first="Alyssa" last="Mcnulty">Alyssa Mcnulty</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Leuzinger, Kahlin" sort="Leuzinger, Kahlin" uniqKey="Leuzinger K" first="Kahlin" last="Leuzinger">Kahlin Leuzinger</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Lai, Huafang" sort="Lai, Huafang" uniqKey="Lai H" first="Huafang" last="Lai">Huafang Lai</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. Huafang.lai@asu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="RBID">pubmed:26614281</idno><idno type="pmid">26614281</idno><idno type="doi">10.1007/978-1-4939-3289-4_4</idno><idno type="wicri:Area/Main/Corpus">000318</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000318</idno><idno type="wicri:Area/Main/Curation">000318</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000318</idno><idno type="wicri:Area/Main/Exploration">000318</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Transient Protein Expression by Agroinfiltration in Lettuce.</title><author><name sortKey="Chen, Qiang" sort="Chen, Qiang" uniqKey="Chen Q" first="Qiang" last="Chen">Qiang Chen</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Dent, Matthew" sort="Dent, Matthew" uniqKey="Dent M" first="Matthew" last="Dent">Matthew Dent</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Hurtado, Jonathan" sort="Hurtado, Jonathan" uniqKey="Hurtado J" first="Jonathan" last="Hurtado">Jonathan Hurtado</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Stahnke, Jake" sort="Stahnke, Jake" uniqKey="Stahnke J" first="Jake" last="Stahnke">Jake Stahnke</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Mcnulty, Alyssa" sort="Mcnulty, Alyssa" uniqKey="Mcnulty A" first="Alyssa" last="Mcnulty">Alyssa Mcnulty</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Life Sciences, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Leuzinger, Kahlin" sort="Leuzinger, Kahlin" uniqKey="Leuzinger K" first="Kahlin" last="Leuzinger">Kahlin Leuzinger</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author><author><name sortKey="Lai, Huafang" sort="Lai, Huafang" uniqKey="Lai H" first="Huafang" last="Lai">Huafang Lai</name><affiliation wicri:level="1"><nlm:affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. Huafang.lai@asu.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225</wicri:regionArea><wicri:noRegion>85225</wicri:noRegion></affiliation></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="eISSN">1940-6029</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Gene Expression (MeSH)</term><term>Genetic Engineering (methods)</term><term>Genetic Vectors (MeSH)</term><term>Lettuce (genetics)</term><term>Lettuce (metabolism)</term><term>Plants, Genetically Modified (MeSH)</term><term>Recombinant Proteins (MeSH)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium tumefaciens (génétique)</term><term>Expression des gènes (MeSH)</term><term>Génie génétique (méthodes)</term><term>Laitue (génétique)</term><term>Laitue (métabolisme)</term><term>Protéines recombinantes (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Vecteurs génétiques (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term><term>Lettuce</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Laitue</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lettuce</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genetic Engineering</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Laitue</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Génie génétique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Genetic Vectors</term><term>Plants, Genetically Modified</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Expression des gènes</term><term>Protéines recombinantes</term><term>Transformation génétique</term><term>Vecteurs génétiques</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26614281</PMID><DateCompleted><Year>2016</Year><Month>10</Month><Day>18</Day></DateCompleted><DateRevised><Year>2016</Year><Month>12</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1385</Volume><PubDate><Year>2016</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol Biol</ISOAbbreviation></Journal><ArticleTitle>Transient Protein Expression by Agroinfiltration in Lettuce.</ArticleTitle><Pagination><MedlinePgn>55-67</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-3289-4_4</ELocationID><Abstract><AbstractText>Current systems of recombinant protein production include bacterial, insect, and mammalian cell culture. However, these platforms are expensive to build and operate at commercial scales and/or have limited abilities to produce complex proteins. In recent years, plant-based expression systems have become top candidates for the production of recombinant proteins as they are highly scalable, robust, safe, and can produce complex proteins due to having a eukaryotic endomembrane system. Newly developed "deconstructed" viral vectors delivered via Agrobacterium tumefaciens (agroinfiltration) have enabled robust plant-based production of proteins with a wide range of applications. The leafy Lactuca sativa (lettuce) plant with its strong foundation in agriculture is an excellent host for pharmaceutical protein production. Here, we describe a method for agroinfiltration of lettuce that can rapidly produce high levels of recombinant proteins in a matter of days and has the potential to be scaled up to an agricultural level.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Qiang</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA. qiang.chen.4@asu.edu.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dent</LastName><ForeName>Matthew</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hurtado</LastName><ForeName>Jonathan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Stahnke</LastName><ForeName>Jake</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McNulty</LastName><ForeName>Alyssa</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>School of Life Sciences, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Leuzinger</LastName><ForeName>Kahlin</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lai</LastName><ForeName>Huafang</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>The Biodesign Institute, Arizona State University, Tempe, AZ, 85225, USA. Huafang.lai@asu.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005818" MajorTopicYN="N">Genetic Engineering</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005822" MajorTopicYN="Y">Genetic Vectors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018545" MajorTopicYN="N">Lettuce</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="Y">Recombinant Proteins</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="Y">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium tumefaciens</Keyword><Keyword MajorTopicYN="N">Agroinfiltration</Keyword><Keyword MajorTopicYN="N">Antibodies</Keyword><Keyword MajorTopicYN="N">Biotechnology</Keyword><Keyword MajorTopicYN="N">Large-scale production</Keyword><Keyword MajorTopicYN="N">Lettuce</Keyword><Keyword MajorTopicYN="N">Plant biology</Keyword><Keyword MajorTopicYN="N">Plant-made pharmaceutics</Keyword><Keyword MajorTopicYN="N">Recombinant protein</Keyword><Keyword MajorTopicYN="N">Transient expression</Keyword><Keyword MajorTopicYN="N">Vaccines</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>11</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>11</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>10</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">26614281</ArticleId><ArticleId IdType="doi">10.1007/978-1-4939-3289-4_4</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Chen, Qiang" sort="Chen, Qiang" uniqKey="Chen Q" first="Qiang" last="Chen">Qiang Chen</name></noRegion><name sortKey="Chen, Qiang" sort="Chen, Qiang" uniqKey="Chen Q" first="Qiang" last="Chen">Qiang Chen</name><name sortKey="Dent, Matthew" sort="Dent, Matthew" uniqKey="Dent M" first="Matthew" last="Dent">Matthew Dent</name><name sortKey="Dent, Matthew" sort="Dent, Matthew" uniqKey="Dent M" first="Matthew" last="Dent">Matthew Dent</name><name sortKey="Hurtado, Jonathan" sort="Hurtado, Jonathan" uniqKey="Hurtado J" first="Jonathan" last="Hurtado">Jonathan Hurtado</name><name sortKey="Hurtado, Jonathan" sort="Hurtado, Jonathan" uniqKey="Hurtado J" first="Jonathan" last="Hurtado">Jonathan Hurtado</name><name sortKey="Lai, Huafang" sort="Lai, Huafang" uniqKey="Lai H" first="Huafang" last="Lai">Huafang Lai</name><name sortKey="Leuzinger, Kahlin" sort="Leuzinger, Kahlin" uniqKey="Leuzinger K" first="Kahlin" last="Leuzinger">Kahlin Leuzinger</name><name sortKey="Mcnulty, Alyssa" sort="Mcnulty, Alyssa" uniqKey="Mcnulty A" first="Alyssa" last="Mcnulty">Alyssa Mcnulty</name><name sortKey="Mcnulty, Alyssa" sort="Mcnulty, Alyssa" uniqKey="Mcnulty A" first="Alyssa" last="Mcnulty">Alyssa Mcnulty</name><name sortKey="Stahnke, Jake" sort="Stahnke, Jake" uniqKey="Stahnke J" first="Jake" last="Stahnke">Jake Stahnke</name><name sortKey="Stahnke, Jake" sort="Stahnke, Jake" uniqKey="Stahnke J" first="Jake" last="Stahnke">Jake Stahnke</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000261 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000261 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26614281
|texte= Transient Protein Expression by Agroinfiltration in Lettuce.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26614281" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |